解磷細菌可在此培養基中利用葡萄糖、酵母浸粉及無(wú)機鹽等成分進(jìn)行生長(cháng)和繁殖,發(fā)酵葡萄糖產(chǎn)酸溶解碳酸鈣,并將難溶性的卵磷脂水解為可溶性磷,菌落周?chē)纬赏该魅Α?/span>
二、培養基配方(g/L):
葡萄糖····················10.0
硫酸銨····················0.5
氯化鈉····················0.3
酵母浸粉····················0.5
氯化鉀····················0.3
硫酸鎂····················0.3
硫酸亞鐵····················0.03
硫酸錳····················0.03
卵磷脂····················0.2
碳酸鈣····················1.0
瓊脂····················15.0
PH:7.0-7.5
三、使用方法:
(1)稱(chēng)取本品28.2g,加熱溶解于1000ml蒸餾水中,121℃高壓滅菌15分鐘;冷卻至45-50℃,將培養基充分搖勻后傾入無(wú)菌平皿,制成平板備用。
(2)挑取新鮮質(zhì)控菌株于無(wú)菌生理鹽水中制成菌懸液,用無(wú)菌滴管滴入制備好的有機磷細菌培養基平板上,每個(gè)平板可均勻接種四個(gè)點(diǎn),置于28-30℃培養2-3天,觀(guān)察有無(wú)透明圈。
四、實(shí)驗結果:
接種以下質(zhì)控菌株,置于28-30℃培養48-72h。
質(zhì)控菌株 |
菌株編號 |
接種量(CFU) |
生長(cháng)情況 |
巨大芽孢桿菌 |
ATCC11107 |
>5000 |
菌落周?chē)型该魅?/span> |
圖1有機磷細菌培養基上的巨大芽孢桿菌生長(cháng)狀態(tài)
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注:本文屬海博生物原創(chuàng ),未經(jīng)允許不得轉載。